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Preparation of stereoregulated antisense oligodeoxyribonucleoside phosphorothioate and interaction with its
Y Tamura1, H Miyoshi, T Yokota
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Nucleosides & Nucleotides
|August 26, 1998
Abstract:
Diastereoisomeric specificity of oligodeoxyribonucleoside phosphorothioate (OPT) in DNA/OPT and RNA/OPT hybrid formation was investigated. The difference in the configuration between RRRR and SSSS was reflected in the conformation and the stability of the DNA/OPT and RNA/OPT hybrids. Therefore, findings of this report rationalize the antisense effect by non-stereoregulated OPT and the difference of diastereoisomerism in susceptibility to RNase H.