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The isolation of genomic DNA from blackcurrant (Ribes nigrum L.)
M Woodhead1, H V Davies, R M Brennan
1Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK. mwoodh@scri.sari.ac.uk
Molecular Biotechnology
|August 27, 1998
Abstract:
A method is described for isolating DNA of high molecular mass (M(r)) from blackcurrant and other softfruit species. Following a hexacethylytimethyl ammonium bromide (CTAB)-based extraction procedure, samples are treated with a glycosidic hydrolase mixture and RNase, and then purified. The suitability of this DNA for Southern analysis and genomic-library construction is demonstrated.