Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genotoxicity in workers exposed to methyl bromide

G M Calvert1, G Talaska, C A Mueller

  • 1Division of Surveillance, Hazard, Evaluations and Field Studies, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 4676 Columbia Parkway, R-21, Cincinnati, OH 45226, USA. jac6@cdc.gov

Mutation Research
|September 12, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Descriptive study of the challenges when implementing an app for patients with neovascular age-related macular degeneration to monitor their vision at home.

BMJ open·2024
Same author

Treatment adherence to olfactory training: a real-world observational study.

Rhinology·2023
Same author

Medicalisation of vaping in the UK? E-cigarette users' perspectives on the merging of commercial and medical routes to vaping.

Perspectives in public health·2023
Same author

Position paper on olfactory dysfunction: 2023

Rhinology·2023
Same author

Lest we forget. Illuminating lived experience of the Covid-19 pandemic and lockdown.

Social science & medicine (1982)·2023
Same author

Extreme weather conditions influence the frequency of epistaxis-related emergency room visits.

Rhinology·2022

Methyl bromide fumigation workers showed potential genotoxic effects, including elevated hprt mutations and oropharyngeal micronuclei. Further research is needed to confirm these findings in human methyl bromide exposure.

Area of Science:

  • Toxicology
  • Environmental Health
  • Human Genetics

Background:

  • Methyl bromide (CAS 74-83-9) is a widely used fumigant with potential health risks.
  • Assessing the genotoxicity of occupational methyl bromide exposure is crucial for worker safety.

Purpose of the Study:

  • To investigate the genotoxicity of in vivo methyl bromide exposure in human subjects.
  • To evaluate micronuclei formation and hprt gene mutations in methyl bromide-exposed workers.

Main Methods:

  • Cross-sectional study involving blood and oropharyngeal cell collection from fumigation workers and referents.
  • Measurement of micronuclei in lymphocytes and oropharyngeal cells.
  • Assessment of hypoxanthine-guanine phosphoribosyl transferase (hprt) gene mutations in lymphocytes.

Related Experiment Videos

Main Results:

  • Elevated hprt variant frequencies observed in non-smoking workers compared to referents, particularly with significant exposure.
  • Increased mean oropharyngeal cell micronuclei in workers versus referents, with similar trends for highly exposed individuals.
  • No consistent differences in lymphocyte micronuclei (kinetochore-negative or -positive) between groups.

Conclusions:

  • Findings suggest a potential association between methyl bromide exposure and genotoxic effects in human lymphocytes and oropharyngeal cells.
  • Study limitations include small sample size and lack of precise exposure quantification.
  • Further investigation into the genotoxicity of methyl bromide in humans is warranted.