Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Capillary electrophoresis chiral separations of basic compounds using cationic cyclodextrin

F Wang1, M G Khaledi

  • 1Department of Chemistry, North Carolina State University, Raleigh 27695-8204, USA.

Electrophoresis
|October 7, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential modulation of proliferation in the neocortical ventricular and subventricular zones.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2000
Same author

Preconditioning with cortical spreading depression decreases intraischemic cerebral glutamate levels and down-regulates excitatory amino acid transporters EAAT1 and EAAT2 from rat cerebal cortex plasma membranes.

Journal of neurochemistry·2000
Same author

Proteomic analysis of the atrophying rat soleus muscle following denervation.

Electrophoresis·2000
Same author

Complex formation between hepatitis C virus core protein and p21Waf1/Cip1/Sdi1.

Biochemical and biophysical research communications·2000
Same author

Modelling the cumulative risk for a false-positive under repeated screening events.

Statistics in medicine·2000
Same author

Two-headed binding of a processive myosin to F-actin.

Nature·2000
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

Cationic cyclodextrins (QA-beta-CD) effectively separate basic enantiomers using counter-electroosmotic flow. This method reduces analyte-cyclodextrin complexation, improving chiral separation resolution and eliminating peak tailing for cationic compounds.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chiral Separations

Background:

  • Chiral separation of basic enantiomers presents challenges due to strong analyte-cyclodextrin complexation with conventional CDs.
  • Peak tailing and poor resolution often occur with cationic compounds using neutral or anionic cyclodextrins.

Purpose of the Study:

  • To investigate the efficacy of cationic cyclodextrins (QA-beta-CD) for chiral separations of basic enantiomers.
  • To explore the impact of counter-electroosmotic flow (counter-EOF) on chiral separation performance.
  • To optimize separation conditions, including pH and QA-beta-CD concentration.

Main Methods:

  • Chiral capillary electrophoresis utilizing quaternary ammonium beta-cyclodextrin (QA-beta-CD) as the chiral selector.
  • Implementation of counter-electroosmotic flow (counter-EOF) conditions.

Related Experiment Videos

  • Systematic variation of pH and QA-beta-CD concentration to assess their effects on separation.

    • Main Results:

    • Cationic QA-beta-CD enabled chiral separation of basic enantiomers under counter-EOF conditions.
    • Reduced analyte-CD complexation was observed, facilitating better control over CD concentration.
    • High resolution chiral separations were achieved for tricyclic compounds at pH 8.20.
    • Separation of bicyclic compounds was obtained at pH 11.6 when enantiomers were neutral.

    Conclusions:

    • Cationic cyclodextrins combined with counter-EOF offer a robust strategy for resolving challenging basic enantiomers.
    • This approach mitigates issues associated with strong binding and peak tailing, enhancing separation efficiency.
    • The method demonstrates versatility across different pH values for separating various classes of chiral amines.