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Preparative gel electrophoresis: detection, excision, and elution of protein bands from unstained gels
1Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331, USA.
Analytical Biochemistry
|November 1, 1978
Abstract:
Methods are described for localizing proteins in unstained gels and accurately excising the regions containing them. A gel elutor, which is all glass with Lucite fittings, is also described. The elutor removes proteins from gels with a high yield and concentrates the protein in a small volume. The elutor is simple and very easy to use. A way is presented for avoiding the oxidation of methionine and cysteine during preparative electrophoresis.