Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multianalyte serum analysis using mid-infrared spectroscopy

R A Shaw1, S Kotowich, M Leroux

  • 1Institute for Biodiagnostics, National Research Council of Canada. shaw@ibd.nrc.ca

Annals of Clinical Biochemistry
|October 13, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Universal correlation between H-linear magnetoresistance and T-linear resistivity in high-temperature superconductors.

Nature communications·2024
Same author

Magnetotransport signatures of antiferromagnetism coexisting with charge order in the trilayer cuprate HgBa<sub>2</sub>Ca<sub>2</sub>Cu<sub>3</sub>O<sub>8+δ</sub>.

Nature communications·2022
Same author

Modeling radiative and non-radiative pathways at both the Franck-Condon and Herzberg-Teller approximation level.

The Journal of chemical physics·2021
Same author

Dispersion Aerosol Indirect Effect in Turbulent Clouds: Laboratory Measurements of Effective Radius.

Geophysical research letters·2019
Same author

Influence of Turbulent Fluctuations on Cloud Droplet Size Dispersion and Aerosol Indirect Effects.

Journal of the atmospheric sciences·2019
Same author

Edge-emitting polariton laser and amplifier based on a ZnO waveguide.

Light, science & applications·2018
Same journal

Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

Annals of clinical biochemistry·2026
Same journal

From interpretable NT-proBNP prediction to equitable laboratory stewardship.

Annals of clinical biochemistry·2026
Same journal

Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

Annals of clinical biochemistry·2026
Same journal

Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

Annals of clinical biochemistry·2026
Same journal

Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

Annals of clinical biochemistry·2026
Same journal

Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

Annals of clinical biochemistry·2026
See all related articles

Mid-infrared (mid-IR) spectroscopy of dried serum films offers a promising method for simultaneously quantifying multiple serum analytes. This technique accurately measures key biomarkers like glucose and cholesterol, though it is less precise for low-concentration analytes such as creatinine.

Area of Science:

  • Biomedical Spectroscopy
  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Serum analyte quantitation is crucial for disease diagnosis and monitoring.
  • Traditional clinical chemistry methods can be time-consuming and require significant sample volumes.
  • Developing rapid, simultaneous methods for multiple analytes is a key research area.

Purpose of the Study:

  • To evaluate mid-infrared (mid-IR) spectroscopy of dried serum films for simultaneous quantitation of eight serum analytes.
  • To assess the accuracy and reliability of IR spectroscopy compared to established clinical chemical methods.
  • To determine the feasibility of this technique for routine clinical diagnostics.

Main Methods:

  • Acquisition of infrared transmission spectra from 300 dried serum samples.

Related Experiment Videos

  • Independent analysis of each sample using accepted reference clinical chemical methods.
  • Development and validation of quantitation models based on IR spectra and reference analyses (200 samples for training, 100 for validation).

    • Main Results:

    • High correlation (r ≥ 0.95) and acceptable standard errors (Sy/x) were achieved for total protein, albumin, triglycerides, cholesterol, glucose, and urea.
    • Sy/x values were 2.8 g/L (total protein), 2.2 g/L (albumin), 0.23 mmol/L (triglycerides), 0.28 mmol/L (cholesterol), 0.41 mmol/L (glucose), and 1.1 mmol/L (urea).
    • The method showed lower suitability for creatinine and uric acid due to their typically low serum concentrations.

    Conclusions:

    • Mid-infrared spectroscopy of dried serum films is a viable technique for the simultaneous quantitation of several major serum analytes.
    • The method demonstrates good accuracy and precision for analytes present at moderate to high concentrations.
    • Further optimization may be needed for accurate quantification of low-concentration analytes like creatinine and uric acid using this approach.