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[Antiendotoxin immunity in experimental intestinal dysbacteriosis in mice]
V G Likhoded1, M Iu Iakovlev, N V Likhoded
1Centre Hydrobios, Institute of Human Morphology, Moscow, Russia.
Abstract:
The development of intestinal dysbacteriosis in white mice, caused by the administration of ampyox in large doses, was shown to be accompanied by a considerable decrease in cell-mediated and humoral antiendotoxin immunity, which was manifested by a decrease in the titers of antiendotoxin antibodies and in the content of polymorphonuclear leukocytes, capable of binding endotoxin.
Insights
Large doses of ampyox caused intestinal dysbacteriosis in mice, significantly reducing their antiendotoxin immunity. This impairment involved lower antibody levels and fewer endotoxin-binding polymorphonuclear leukocytes.
Area of Science:
- Microbiology
- Immunology
- Toxicology
Background:
- Intestinal dysbacteriosis, an imbalance in gut microbiota, can impact host immunity.
- Endotoxins, particularly lipopolysaccharides from Gram-negative bacteria, are potent immune stimulators.
- Ampyox is an antimicrobial agent whose effects on host immunity require investigation.
Purpose of the Study:
- To investigate the impact of high-dose ampyox administration on intestinal microbiota and host antiendotoxin immunity in a murine model.
- To assess changes in humoral and cell-mediated immune responses related to endotoxin challenge.
Main Methods:
- White mice were administered large doses of ampyox to induce intestinal dysbacteriosis.
- Cell-mediated immunity was assessed by evaluating polymorphonuclear leukocytes' ability to bind endotoxin.
- Humoral immunity was evaluated by measuring antiendotoxin antibody titers.
Main Results:
- Ampyox administration led to significant intestinal dysbacteriosis.
- A considerable decrease in cell-mediated antiendotoxin immunity was observed.
- Humoral antiendotoxin immunity was also markedly reduced, evidenced by lower antiendotoxin antibody titers.
Conclusions:
- High-dose ampyox induces intestinal dysbacteriosis and impairs both cell-mediated and humoral antiendotoxin immunity in mice.
- The reduction in polymorphonuclear leukocytes capable of binding endotoxin highlights a key mechanism of immune suppression.
- These findings suggest potential risks associated with high-dose ampyox use regarding host defense against endotoxins.