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Updated: Aug 16, 2026
Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
Published on: August 5, 2012
Contusion of skeletal muscle increases leukocyte-endothelial cell interactions: an intravital-microscopy study in
W A Menth-Chiari1, W W Curl, E Rosencrance
1Department of Orthopaedic Surgery, Wake Forest University School of Medicine, Baptist Medical Center, Winston-Salem, North Carolina 27157-1070, USA.
Objective:
To investigate the relationship between secondary muscle damage after contusion and the interactions between leukocytes and endothelial cells, which are essential steps in secondary inflammatory response.
Methods:
In a randomized animal study, rats were chronically instrumented with dorsal skinfold microvascular chambers and exposed to standardized contusion or sham contusion. Leukocyte rolling and adherence in postcapillary venules before and after muscle contusion or sham contusion were quantitated using in vivo microscopy.
Results:
The number of rolling leukocytes in the postcapillary venules before contusion was low. At 300 minutes after contusion, the number of rolling and adherent leukocytes in the striated muscle microvasculature was increased significantly (p < or = 0.05) compared with either the baseline precontusion condition or the control group at the same time.
Conclusion:
In the mid-term to long-term stages of skeletal muscle injury associated with contusion, a significant portion of tissue damage is secondary to leukocyte-endothelial cell interactions.
