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[26S-ribonucleoprotein complex (26S-proteasome) directly interacts with fibrillar actin]
V E Galkin1, L V Turoverova, I M Konstantinova
1Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Tsitologiia
|October 30, 1998
Abstract:
26S-complex was purified from rat liver cells by centrifugation in sucrose gradients and ion exchange chromatography. The ability of 26S-proteasome to interact with fibrillar actin (F-actin) was examined by rapid cosidementation of these particles with F-actin in isokinetic sucrose gradients. It was shown that direct interaction occurred only at a low ATP concentration.