Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods for subtyping lupus anticoagulants

T Exner1

  • 1Gradipore Ltd, Sydney, Australia.

Lupus
|November 14, 1998
PubMed
Summary
This summary is machine-generated.

Identifying lupus anticoagulant cofactor dependence is challenging. Two simple tests showed limited success in differentiating beta2-glycoprotein 1 (beta2GP1) or prothrombin dependence, indicating a need for further research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Women's Perceptions of HIV- and Sexuality-Related Stigma in Relation to PrEP: Qualitative Findings from the Masibambane Study, Durban, South Africa.

AIDS and behavior·2022
Same author

Setting the stage for next-generation risk assessment with non-animal approaches: the EU-ToxRisk project experience.

Archives of toxicology·2020
Same author

Clotting test results correlate better with DOAC concentrations when expressed as a "Correction Ratio"; results before/after extraction with the DOAC Stop reagent.

Thrombosis research·2019
Same author

Simple method for removing DOACs from plasma samples.

Thrombosis research·2018
Same author

Infusion of foam sclerosants results in a distance-dependent procoagulant activity, haemoconcentration and elevation of D-dimer levels.

Phlebology·2013
Same author

In vitro effects of detergent sclerosants on clot formation and fibrinolysis.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2010
Same journal

Association of GPX4 rs713041 and rs4807542 polymorphisms and serum GPX4 levels in Chinese patients with systemic lupus erythematosus.

Lupus·2026
Same journal

Giant cell myocarditis in an 18-year-old patient with new-onset systemic lupus erythematosus: A fatal case of rapidly progressing heart failure.

Lupus·2026
Same journal

An evidence-based specialist nursing protocol for children with hypoprothrombinemia-lupus anticoagulant syndrome (HLAS) and its application research.

Lupus·2026
Same journal

T-bet expression in B cell subsets: Association with T peripheral helper cells and clinical activity in systemic lupus erythematosus.

Lupus·2026
Same journal

Autonomic dysfunction in systemic lupus erythematosus and systemic sclerosis.

Lupus·2026
Same journal

Recent advances in the management of pediatric neuropsychiatric lupus from conventional therapies to novel immunomodulators.

Lupus·2026
See all related articles

Area of Science:

  • Clinical immunology
  • Hematology
  • Diagnostic laboratory medicine

Background:

  • Lupus anticoagulants (LA) are associated with thrombosis.
  • Distinguishing between beta2-glycoprotein 1 (beta2GP1)-dependent and prothrombin-dependent LA is clinically relevant.
  • Current diagnostic methods may lack specificity in identifying cofactor dependence.

Purpose of the Study:

  • To evaluate two novel, simple procedures for identifying beta2-glycoprotein 1 (beta2GP1) or prothrombin cofactor dependence in lupus anticoagulants (LA).
  • To assess the utility of these tests in differentiating LA subtypes using common clotting assays.

Main Methods:

  • A beta2GP1-deficient plasma mixing test (1:4 ratio) was compared to using normal plasma.
  • A bovine plasma mixing test (1:4 ratio) was evaluated using the Kaolin Clotting Time (KCT).

Related Experiment Videos

  • The effects of these tests on LA detection in KCT, Dilute Russell Viper Venom Time (DRVVT), and Activated Partial Thromboplastin Time (APTT) were analyzed.

    • Main Results:

    • beta2GP1 deficiency partially reduced, but did not eliminate, most LA detected by KCT, DRVVT, and APTT.
    • Bovine plasma showed a similar correcting effect on LA across all three tested clotting assays.
    • A prothrombin antibody prolonged clotting times similarly in all three assays, and LA patient plasmas exhibited significant heterogeneity.

    Conclusions:

    • The evaluated simple procedures demonstrated limited ability to reliably differentiate beta2GP1-dependent from prothrombin-dependent LA.
    • DRVVT and KCT tests, in their current forms, do not appear to effectively discriminate between these LA subtypes.
    • Further investigation is needed to develop accurate diagnostic tools for LA cofactor dependence, and the clinical significance of these tests remains uncertain.