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A mouse model of acute bacterial rhinosinusitis
K Bomer1, A Brichta, F Baroody
1Section of Otolaryngology-Head and Neck Surgery, University of Chicago, Ill 60637, USA.
Objective:
To develop a mouse model of acute bacterial rhinosinusitis.
Design:
Study mice (C57BL6/J) were inoculated intranasally with Streptococcus pneumoniae, ATCC 49619 suspended in trypticase soy broth, and controls were inoculated with trypticase soy broth alone. After 2, 5, or 14 days, intranasal cultures were obtained and mice were killed. The sinuses were prepared for histological investigation.
Setting:
Animal care facility at a tertiary, academic institution.
Method:
The histological sections of the sinuses were examined in a blinded manner for the percentage of sinus cavity occupied by neutrophil clusters, and for the number of neutrophils per square millimeter of sinus mucosa.
Results:
Infected mice killed at 5 days had significantly more sinus area occupied by neutrophil clusters, significantly more neutrophils within the mucosa, and significantly more S pneumoniae growth in the intranasal cultures compared with controls (15/15 vs 0/6; P<.01). The amount of inflammation had decreased at 2 weeks.
Conclusion:
Streptococcus pneumoniae induces acute bacterial rhinosinusitis in C57BL6/4 mice as measured by culture and influx of neutrophils, and can be used as a model of acute bacterial rhinosinusitis.
Insights
This study developed a mouse model for acute bacterial rhinosinusitis using Streptococcus pneumoniae. The model successfully demonstrated bacterial infection and neutrophil influx in mouse sinuses.
Area of Science:
- Microbiology
- Immunology
- Pathology
Background:
- Acute bacterial rhinosinusitis (ABRS) is a common condition with significant patient impact.
- Developing reliable animal models is crucial for understanding ABRS pathogenesis and testing therapeutics.
Purpose of the Study:
- To establish a reproducible mouse model of acute bacterial rhinosinusitis.
- To characterize the host response to Streptococcus pneumoniae infection in the murine sinus.
Main Methods:
- C57BL6/J mice were intranasally inoculated with Streptococcus pneumoniae.
- Control mice received trypticase soy broth alone.
- Sinus tissues were analyzed histologically for neutrophil infiltration and cultured for bacterial load at 2, 5, and 14 days post-inoculation.
Main Results:
- Mice infected with S. pneumoniae showed significantly increased neutrophil clusters and mucosal neutrophils compared to controls at 5 days.
- Significant S. pneumoniae growth was detected in infected mice.
- Inflammation markers decreased by the 2-week time point.
Conclusions:
- Streptococcus pneumoniae successfully induces acute bacterial rhinosinusitis in C57BL6/J mice.
- The model is validated by culture and neutrophil influx.
- This mouse model can be utilized for further ABRS research.