[Physiologic-biochemical features of Morganella under various conditions of cultivation]

The parameters of the cultivation process and the content of protein and polysaccharide in M.morganii cells grown under the conditions of submerged cultivation in 2 nutrient media having different composition were studied. The study revealed that the highest values of specific growth rate and the maximum yield of biomass were observed during cultivation under the conditions of medium values of oxygen concentration in the medium (pO2 = 20-30%). The content of protein in bacterial cells was practically independent on the growth phase of the culture, with the exception of cultivation processes in carbohydrate-free media with the use of "old" seed material (48-hours agar culture) when protein content in the cells was higher in the exponential phase. The content of polysaccharide in bacterial cells, its maximum level observed at the exponential phase, considerably dropped at the growth deceleration and stationary phases.