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Updated: Aug 10, 2026
12:37
Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
[Database on nucleotide sequences used as primers of microorganisms]
B N Mishan'kin1, N L Volokhonskaia, E A Moskvitina
1Research Institute for Plaque Control, Rostov-on-Don, Russia.
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|November 24, 1998
Summary
A new database, "Primers of microorganisms," organizes oligonucleotide sequences for polymerase chain reaction (PCR) diagnostics. It covers 20 bacterial genera and 6 viral families, aiding microbial identification.
Area of Science:
- Microbiology
- Molecular Biology
- Bioinformatics
Context:
- Polymerase Chain Reaction (PCR) is a cornerstone of molecular biology and diagnostics.
- Accurate and accessible primer information is crucial for reliable PCR-based identification of microorganisms.
- Existing resources may lack comprehensive or systematized data on primers for diverse bacterial and viral targets.
Purpose:
- To create a centralized, systematized database of oligonucleotide sequences used as primers in PCR.
- To facilitate laboratory diagnostics by providing curated primer information for various pathogens.
- To support researchers and diagnosticians by consolidating data from bibliographic sources.
Summary:
- The
- Primers of microorganisms
- database has been established, compiling oligonucleotide sequences for PCR applications.
- It encompasses primers for the laboratory diagnostics of 20 bacterial genera and 6 viral families.
- The database contains 145 primer pairs and references 530 sources, with data spanning 10 years (1987-1996) and ongoing updates.
Impact:
- Enhances the efficiency and accuracy of microbial diagnostics through readily available PCR primer data.
- Serves as a valuable resource for researchers developing new diagnostic assays.
- Supports public health by improving the identification of bacterial and viral pathogens.
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