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Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic
T Yajima1, A Yagihashi, H Kameshima
1Department of Laboratory Diagnosis, University School of Medicine, Sapporo 060-8543, Japan.
Clinical Chemistry
|December 4, 1998
Abstract:
We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than that in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determining the amount of hTR in clinical specimens.