Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Accessibility control of variable region gene assembly during T-cell development

B P Sleckman1, C H Bassing, C G Bardon

  • 1Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115, USA.

Immunological Reviews
|December 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment of recurrent giant iris cyst with intracyst administration of mitomycin C.

The British journal of ophthalmology·2000
Same author

Congenital urethrocutaneous fistula.

International journal of urology : official journal of the Japanese Urological Association·2000
Same author

Comparison of water- and land-based exercise in the reduction of state anxiety among older adults.

Perceptual and motor skills·2000
Same author

Developmental expression pattern of the cdo gene.

Developmental dynamics : an official publication of the American Association of Anatomists·2000
Same author

Laparoscopic esophagomyotomy with dor anterior fundoplication in a child with achalasia.

Surgical laparoscopy, endoscopy & percutaneous techniques·2000
Same author

Meconium peritonitis in utero.

Pediatric surgery international·2000

T-cell development involves precise gene assembly for T-cell antigen receptors (TCRs). This study investigates how accessibility of TCR gene loci is regulated during T-cell development to ensure proper receptor expression.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • T-cell development requires ordered assembly and expression of T-cell antigen receptor (TCR) genes.
  • T cells differentiate into alpha beta or gamma delta lineages based on TCR expression.
  • TCR gene assembly involves site-specific recombination, tightly regulated during T-cell development.

Purpose of the Study:

  • To investigate the regulation of recombination and expression of TCR genes.
  • To analyze the role of cis-acting elements in TCR gene regulation.
  • To understand the regulation of TCR beta and TCR alpha/delta loci accessibility during T-cell development.

Main Methods:

  • Development of experimental approaches to assess cis-acting element roles.
  • Analysis of TCR gene recombination and expression.

Related Experiment Videos

  • Investigation of locus accessibility during T-cell development.

    • Main Results:

    • TCR beta, gamma, and delta variable region genes assemble before TCR alpha variable region genes.
    • TCR beta variable region gene assembly is regulated by allelic exclusion.
    • TCR alpha and delta variable region genes assemble at distinct developmental stages and express in separate T-cell lineages.

    Conclusions:

    • Regulation of TCR alpha/delta locus rearrangement and expression is complex.
    • Accessibility of TCR beta and TCR alpha/delta loci is a key regulatory mechanism in T-cell development.
    • Experimental approaches were established to study TCR gene regulation.