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Efficient gene transfer to human squamous cell carcinomas by the herpes simplex virus type 1 amplicon vector
J F Carew1, H Federoff, M Halterman
1Department of Head and Neck Surgery, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA.
American Journal of Surgery
|January 5, 1999
Summary
Herpes simplex virus (HSV) vectors efficiently transfer genes into human squamous cell carcinoma (SCC) cells. Selective intra-arterial perfusion enhances gene delivery in vitro and in vivo for SCC treatment.
Area of Science:
- Oncolytic virology
- Gene therapy
- Cancer research
Background:
- Evaluating herpes simplex virus (HSV) mediated gene transfer efficiency.
- Focus on human squamous cell carcinoma (SCC) cell lines.
- In vitro and in vivo delivery via selective intra-arterial perfusion.
Purpose of the Study:
- Assess HSV gene transfer efficiency in human SCC.
- Investigate in vitro and in vivo performance.
- Determine effectiveness of intra-arterial perfusion delivery.
Main Methods:
- Exposed human head and neck SCC to HSV-LacZ and HSV-interleukin-2 (IL-2).
- Assessed gene transfer via X-gal staining and IL-2 via ELISA.
- Perfused hamster cheek pouch tumors with HSV vectors via external carotid artery.
Main Results:
- Gene transfer rates exceeded 50% at 5 viral particles/tumor cell.
- Achieved IL-2 levels of 287-424 ng/million cells at 2 viral particles/tumor cell.
- Selective intra-arterial perfusion yielded 45.8 pg/g tumor IL-2 levels.
Conclusions:
- HSV amplicon vectors demonstrate efficient gene transfer.
- Effective in vitro for human head and neck SCC cell lines.
- Successful in vivo gene transfer via selective intra-arterial perfusion.