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Wavelength specific activation of PI 3-kinase by UVB irradiation
Y Kabuyama1, M Hamaya, Y Homma
1Department of Biomolecular Science, Institute of Biomedical Sciences, Fukushima Medical College, Japan.
Abstract:
We investigated the effect of UVB irradiation on phospholipid metabolism using normal human diploid skin fibroblasts. When cells were exposed to monochromatic UV light, the intracellular PIP3 level was increased within 1 min in a wavelength-specific manner. The increase was most marked in the UVB range and was inhibited by a PI 3-kinase inhibitor, tyrosine kinase inhibitor, or antioxidant reagents. Furthermore, the decrease of cell viability induced by UVB irradiation was significantly blocked by wortmannin. These results suggest that PI 3-kinase activation is involved in novel cellular responses specific for UVB that lead to cell death.