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Related Experiment Videos

Differential implication of StAR and P450c17 in TGFbeta1-induced decrease of adrenocortical steroidogenesis

C Brand1, S Bailly, G Defaye

  • 1INSERM Unit 244, Department of Molecular and Structural Biology, CEA/G, Grenoble, France.

Endocrine Research
|January 15, 1999
PubMed
Summary
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Transforming growth factor beta 1 (TGFbeta1) affects steroidogenic acute regulatory protein (StAR) and P450c17 expression in bovine adrenocortical cells. StAR appears to be the primary in vivo target of TGFbeta1 in the adrenal cortex.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Adrenocortical fasciculata cells dedifferentiate in primary culture.
  • Transforming growth factor beta (TGFbeta) has known targets in these cells: StAR and P450c17.

Purpose of the Study:

  • Investigate TGFbeta1 regulation of StAR and P450c17 expression over time in primary cell culture.
  • Determine the primary in vivo target of TGFbeta1 in the adrenal cortex.

Main Methods:

  • Primary culture of bovine adrenocortical fasciculata cells.
  • Treatment with TGFbeta1 and ACTH at different time points (Day 1 and Day 4).
  • Analysis of StAR and P450c17 mRNA expression levels.

Main Results:

Related Experiment Videos

  • On Day 1, TGFbeta1 decreased basal StAR and P450c17 expression. In the presence of ACTH, TGFbeta1 down-regulated StAR but not P450c17 mRNA.
  • On Day 4, TGFbeta1 down-regulated both StAR and P450c17 mRNA in the absence and presence of ACTH.
  • These time-dependent effects suggest differential regulation of these genes by TGFbeta1.

    • Conclusions:

    • TGFbeta1 exhibits time-dependent regulation of StAR and P450c17 expression in cultured bovine adrenocortical cells.
    • StAR is identified as the most likely major in vivo target of TGFbeta1 action within the adrenal cortex.