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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
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Published on: January 19, 2011

Quantitative imaging of the green fluorescent protein (GFP)

D W Piston1, G H Patterson, S M Knobel

  • 1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA.

Methods in Cell Biology
|January 19, 1999
PubMed
Abstract

No abstract available in PubMed .

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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...