Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA enzymes

D Sen1, C R Geyer

  • 1Institute of Molecular Biology and Biochemistry Department of Chemistry Simon Fraser University Burnaby British Columbia V5A 1S6 Canada. sen@sfu.ca

Current Opinion in Chemical Biology
|January 23, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome Sequence of <i>Dermacoccus</i> Strain Tok2021, a Soil Actinobacterium Isolated from a Pinus radiata Forest.

Microbiology resource announcements·2022
Same author

Occupational health management of work-related stress: guidelines versus practice.

Occupational medicine (Oxford, England)·2021
Same author

Role of free volumes and segmental dynamics on ion conductivity of PEO/LiTFSI solid polymer electrolytes filled with SiO<sub>2</sub> nanoparticles: a positron annihilation and broadband dielectric spectroscopy study.

Physical chemistry chemical physics : PCCP·2021
Same author

TRPV2 POLYMORPHISMS INCREASE OR REDUCE THE RISK OF TYPE 2 DIABETES - HASHIMOTO THYROIDITIS COMORBIDITY.

Acta endocrinologica (Bucharest, Romania : 2005)·2020
Same author

Aquaporin-4 IgG antibody-related disorders in patients with juvenile systemic lupus erythematosus.

Lupus·2019
Same author

Probing the effect of a room temperature ionic liquid on phospholipid membranes in multilamellar vesicles.

European biophysics journal : EBJ·2018
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

DNA enzymes have advanced significantly, broadening their catalytic abilities and demonstrating cofactor-independent RNA cleavage. The most efficient RNA-cleaving nucleic acid enzyme is now a DNA enzyme.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • DNA enzymes, or deoxyribozymes, are increasingly recognized for their catalytic potential beyond simple genetic material.
  • Recent research has expanded the understanding of nucleic acid enzyme activities and catalytic mechanisms.

Purpose of the Study:

  • To highlight the recent advancements and broadened scope of DNA enzyme research.
  • To showcase novel DNA enzyme activities, including cofactor utilization and RNA cleavage.
  • To emphasize the practical implications and efficiency of DNA enzymes in catalysis.

Main Methods:

  • Characterization of novel DNA enzyme activities.
  • Investigation of cofactor requirements for DNA enzyme catalysis.
  • Assays to determine the efficiency of RNA-cleaving DNA enzymes.

Related Experiment Videos

Main Results:

  • Discovery of the first DNA enzyme utilizing an amino acid cofactor.
  • Identification of DNA enzymes capable of cleaving RNA without external cofactors.
  • Development of a DNA enzyme exhibiting superior RNA cleavage efficiency compared to previously known nucleic acid enzymes.

Conclusions:

  • DNA enzymes represent a rapidly advancing field with a diverse catalytic repertoire.
  • The development of cofactor-independent and highly efficient RNA-cleaving DNA enzymes has significant practical applications.
  • DNA enzymes are moving from curiosities to powerful tools in molecular catalysis.