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A site-specific endonuclease from thermophilic strain Bacillus species ZE is a ClaI isoschizomer
E N Zheleznyakova1, L A Zheleznaya, I V Svad'bina
1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia.
Biochemistry. Biokhimiia
|January 23, 1999
Abstract:
Strain Bacillus species ZE with a maximal yield of the ClaI isoschizomer was chosen from 12 natural thermophilic strains producing ClaI isomers. The yield is 110 times higher than that of the ClaI prototype endonuclease from Caryophanon latum L. The enzyme is sensitive to DNA dam-methylation and is highly stable under storage.