[A new method for wound age estimation. Histoelectrofocussing (author's transl)]

Histoelectrofocussing is a combination of the usual histochemical methods of wound age estimation with biochemical procedure. Histological sections from skin wounds were directly subjected to isoelectric focussing. The enzymes being extracted and separated were detected by means of histochemical approach. The alterations of the activity of different enzyme fractions can be calculated quantitatively by densitometry. It is possible to differentiate between enzymes of the common skin and those with a topographic connection to the wound edge.