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Published on: February 20, 2015
[The detection of basement membrane and dendritic cells in laryngeal carcinoma]
1Department of Otolaryngology, Sir Run Run Show Hospital, Zhejiang Medical University, Hangzhou 310016.
Insights
Basement membrane (BM) integrity and dendritic cell (DC) presence are valuable prognostic indicators for laryngeal carcinoma. Continuous BM and positive DC correlate with better survival rates in patients.
Area of Science:
- Oncology
- Immunology
- Pathology
Context:
- Laryngeal carcinoma prognosis requires reliable biomarkers.
- Basement membrane (BM) integrity and dendritic cell (DC) infiltration are implicated in tumor progression.
- Immunohistochemical analysis provides insights into tissue microenvironment changes.
Purpose:
- To investigate the prognostic value of basement membrane (BM) status and dendritic cell (DC) presence in laryngeal carcinoma.
- To correlate BM integrity and DC levels with clinicopathological features and patient survival.
- To evaluate the utility of these markers for prognostic analysis in laryngeal cancer.
Summary:
- Immunohistochemistry was used to assess BM (laminin, type IV collagen) and DC (S-100 protein) in 68 laryngeal carcinoma cases and 21 controls.
- Discontinuous BM and absence of DC were associated with poorer differentiation and advanced stages of laryngeal carcinoma.
- Continuous BM and positive DC presence significantly correlated with higher five-year survival rates, with the best prognosis observed in patients exhibiting both continuous BM and positive DC.
Impact:
- Identifies basement membrane (BM) status and dendritic cell (DC) presence as significant prognostic factors for laryngeal carcinoma.
- Suggests that immunohistochemical detection of BM and DC can aid in stratifying patients for treatment and predicting outcomes.
- Highlights the potential of these markers for improving prognostic accuracy in clinical practice.
Abstract:
The state of basement membrane(BM) and dendritic cells(DC) were observed by immunohistochemical methods with the use of anti-laminin antibody, anti-type IV collagen antibody, anti-S-100 protein antibody in 68 cases of laryngeal carcinoma and 21 cases of non-cancer laryngeal tissues. The results showed that among 68 patients with laryngeal carcinoma 39 cases had discontinuous BM. A continuous, linear BM was found mostly in normal larynx, atypia hyperplasia and papilloma, only 3 cases had discontinuous BM. 43 positive DC were detected in 68 cases of laryngeal carcinoma. There were rarely positive DC in non-cancer laryngeal tissues. The poorer the differentiation of carcinoma, the more the discontinuous BM and the lesser the number of DC. The discontinuous BM and DC(-) were seen more in advanced stages of carcinoma. The five-year survival rate in patients with continuous BM was significantly higher than that in patients with discontinuous BM, the five-year survival rate in patients with DC positive was also higher than that in patients with DC negative; the survival rate was highest in patients with continuous BM and DC positive. Our results indicated that the detection of the state of BM and dendritic cells were of value to the prognostic analysis in patients with laryngeal carcinoma.
