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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Published on: March 5, 2019
New developments in flow cytometric analyses of lymphocyte markers
T J Kipps1, G Meisenholder, B A Robbins
1Department of Medicine, University of California, San Diego, La Jolla.
Insights
This guide explains clinical flow cytometry, a vital diagnostic tool. It covers technical methods for setting up and running a lab, focusing on chronic lymphoproliferative diseases.
Area of Science:
- Clinical Laboratory Science
- Immunology
- Hematology
Background:
- Clinical flow cytometry is a rapidly advancing field with significant clinical and investigative applications.
- Accurate diagnosis and monitoring of diseases rely on robust laboratory methodologies.
Purpose of the Study:
- To provide a practical overview of the technical and methodologic aspects of clinical flow cytometry.
- To discuss the application of flow cytometry in diagnosing and monitoring chronic lymphoproliferative diseases.
- To serve as a guide for establishing and maintaining clinical flow cytometry laboratories.
Main Methods:
- Overview of fundamental features and practical aspects of clinical flow cytometry.
- Discussion of technical and methodologic considerations for laboratory setup and operation.
- Exploration of flow cytometry applications in specific disease contexts.
Main Results:
- Provides a comprehensive understanding of clinical flow cytometry principles and practices.
- Highlights the utility of flow cytometry in the diagnosis and management of lymphoproliferative disorders.
- Offers practical guidance for laboratory personnel.
Conclusions:
- Clinical flow cytometry is an essential tool for modern diagnostics and research.
- Proper implementation of technical and methodologic standards ensures reliable results.
- This article serves as a valuable resource for professionals in the field.
Abstract:
This article addresses several fundamental features of clinical flow cytometry. A practical overview of the technical and methodologic aspects of this fast-growing and increasingly important new clinical and investigative field is provided. The use of flow cytometry for the diagnosis and monitoring of various chronic lymphoproliferative diseases also is discussed. This article may serve as a useful guide to those seeking to set up or maintain a clinical flow cytometry laboratory.
