[To study the expression of dendritic cell phenotypic antigen in cervical lymph nodes in hypopharyngeal carcinoma]
Xiaoyan Li1, Tadashi Nakashima, Pin Dong
1Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University, First People's Hospital, China.
Insights
Dendritic cell (DC) phenotypic antigens in hypopharyngeal carcinoma lymph nodes differ based on metastasis and prognosis. Lower expression of S-100, CD1a, and CD83+ DCs correlates with metastasis and poorer survival, indicating their prognostic value.
Area of Science:
- Immunology
- Oncology
- Pathology
Context:
- Dendritic cells (DCs) are crucial for antigen presentation and immune response.
- Hypopharyngeal carcinoma is a significant head and neck cancer with variable prognosis.
- Understanding immune cell markers in the tumor microenvironment is vital for predicting outcomes.
Purpose:
- To investigate the expression of DC phenotypic antigens (S-100, CD1a, CD83) in cervical lymph nodes of hypopharyngeal carcinoma patients.
- To evaluate the association between DC phenotypic antigen expression, lymph node metastasis, and patient prognosis.
Summary:
- Reduced numbers of S-100+, CD1a+, and CD83+ DCs were observed in lymph node metastasis compared to non-metastatic cases.
- CD83+ DCs were less abundant in cancerous nests than in peritumor areas within metastatic lymph nodes.
- Higher CD83+ DC counts were associated with improved survival, and CD45RO+ T cells predominantly surrounded CD83+ DCs.
Impact:
- DC phenotypic antigen expression provides significant differences in various lymph node areas, suggesting a role in anti-tumor immunity.
- These DC markers are valuable indicators for predicting the prognosis of hypopharyngeal carcinoma patients.
- This research aids in developing targeted immunotherapies and improving patient stratification.
Objective:
Dendritic cells express many cell phenotypic antigens, they have different special function in the process of antigen present. The purpose of this study was to determine some DC phenotypic antigen in cervical lymphnodes in hypopharyngeal carcinoma and evaluate the relationship between the DC phenotypic antigen expression in hypopharyngeal carcinoma metastasis and prognosis.
Method:
Envision HIS method was performed to detect the cell surface markers S-100, CD1a and CD83. 144 cervical lymph nodes obtained from 37 cases of hypopharyngeal carcinoma were evaluated. Furthermore, the relationship between CD45RO+ T lymphocytes and dendritic cells was observed.
Result:
The number of S-100+ DCs, CD1a+ DCs and CD83+ DCs in the cases of lymphnode metastasis were fewer than those in non-lymphnode metastasis. There is a significant metastasis difference in statistics between lymphnode metastasis groupe and non-lymphnode metastasis group. The number of CD83+ DCs located at cancerous nest area were fewer than those at peritumor area in lymphnode metastasis groupe. There is a significant difference in statistics between them. The number of CD83+ DCs in the cases of survival group were more than those in obituary group. CD45RO+ T cells mainly distribute around CD83+ DCs.
Conclusion:
The expression of dendritic cells phenotypic antigens were significant difference in different areas in lymphnodes, may play difference role in tumor immunity against hypopharyngeal carcinoma. Dendritic cells phenotypic antigens were the important markers to predict the prognosis of the patients with hypopharyngeal carcinoma.
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