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Updated: Jul 13, 2026
Quality-Controlled Sputum Analysis by Flow Cytometry
Published on: August 9, 2021
Quality control in clinical flow cytometry
1Genzyme Genetics, 5300 McConnell Avenue, Los Angeles, CA 90066, USA. teri.oldaker@genzyme.com
Insights
Flow cytometry immunophenotyping precisely identifies blood cells and cancers. Standardized procedures and quality control are crucial for accurate diagnostic and prognostic information in patient care.
Area of Science:
- Hematology
- Immunology
- Clinical Diagnostics
Background:
- Flow cytometry immunophenotyping is a complex technology for analyzing leukocytes.
- It is vital for characterizing normal lymphocyte subsets and hematologic neoplasms.
- Irreplaceable samples like bone marrow and fresh tissue are often used.
Purpose of the Study:
- To highlight the importance of flow cytometry immunophenotyping in hematologic diagnostics.
- To emphasize the critical role of sample handling and analysis in patient treatment decisions.
- To underscore the necessity of standardized procedures and quality control.
Main Methods:
- Enumeration and characterization of leukocytes using flow cytometry.
- Analysis of diverse biological samples including peripheral blood, bone marrow, and fresh tissue.
- Comprehensive review of preanalytic, analytic, and postanalytic processes.
Main Results:
- Accurate immunophenotyping yields critical diagnostic and prognostic information.
- Standardized protocols ensure reliable enumeration and characterization of cell populations.
- Quality assurance programs are essential for reproducible results.
Conclusions:
- Optimized and standardized flow cytometry immunophenotyping is essential for accurate patient diagnosis.
- Rigorous quality control across all process stages is critical for reliable results.
- This technology significantly impacts treatment decisions for hematologic conditions.
Abstract:
Immunophenotyping by flow cytometry is a robust and highly complex technology used in the enumeration and characterization of leukocytes, including normal lymphocyte subsets and hematologic neoplasms. Samples consist of peripheral blood and many times irreplaceable samples, such as bone marrow and fresh tissue. These samples are collected, transported, prepared, analyzed, and interpreted, resulting in diagnostic and prognostic information. Such information is critical to treatment decisions for patients. In order to obtain accurate and reproducible results, it is essential to have optimized and standardized procedures, rigorous quality control, and assurance programs encompassing preanalytic, analytic, and postanalytic processes.
