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Published on: August 2, 2017
Study for the improvement of umbilical cord blood sampling using a new trial apparatus
Naoki Masaoka1, Masako Morooka, Yoshiyuki Nakajima
1Department of Obstetrics and Gynecology, Tokyo Women's Medical University Yachiyo Medical Center, Chiba, Japan.
Insights
The trial umbilical cord blood (UCB) sampling bag collected significantly higher UCB volumes compared to traditional bags. This new method shows promise for UCB transplantation, with no compromise in UCB quality.
Area of Science:
- Reproductive Medicine
- Hematology
- Transplantation Science
Background:
- Umbilical cord blood (UCB) is a valuable source for hematopoietic stem cell transplantation.
- Efficient collection methods are crucial for maximizing UCB yield and quality.
- Evaluating new collection devices is essential for improving transplantation outcomes.
Purpose of the Study:
- To assess the efficacy of a trial umbilical cord blood (UCB) sampling bag for unrelated cord blood transplantation.
- To compare UCB collection volume and quality between a trial bag and a traditional method.
- To determine the potential usefulness of the trial bag in clinical settings.
Main Methods:
- A comparative study involving 100 vaginal deliveries.
- UCB collected using either a traditional Kawasumi bag (50 cases) or a trial NIPRO bag (50 cases).
- Comparison of UCB volume; detailed quality analysis (cell counts, CD34+ cells, CFU-GM, bacterial contamination) in matched subgroups.
Main Results:
- The trial sampling bag yielded significantly higher UCB volumes than the traditional bag (P < 0.05).
- This volume difference was more pronounced later in the study period.
- No significant differences were observed in UCB quality parameters between the two collection methods.
Conclusions:
- The trial UCB sampling bag demonstrates potential for increased UCB collection volume.
- The method shows no adverse impact on UCB quality.
- With clinical adaptation, the trial bag may become a valuable tool for collecting UCB for transplantation.
Aim:
The aim of this study was to evaluate the usefulness of the trial umbilical cord blood sampling bag for unrelated cord blood transplantation.
Material And Methods:
Data were obtained from 100 vaginal deliveries. In 50 cases, umbilical cord blood (UCB) was taken with the traditional Kawasumi type UCB sampling bag. In another 50 cases, UCB were taken with trial UCB sampling bag offered by NIPRO Co. We compared the sampling volume between the two groups. Furthermore, 10 cases in each group were matched by sampling volume; we examined the quality of UCB on the number and concentration of nucleated cells, mononuclear cells, CD34+ cells and colony-forming unit granulocyte macrophage and the numbers tested positive for bacteria.
Results:
Whereas there were no significant differences in gestational weeks at sampling, the ratio of primipara women to multipara women, maternal age, and neonatal weight between the two groups, the sampling UCB volumes with the trial sampling bag were significantly higher than those with traditional sampling bags (P < 0.05). In addition, this phenomenon was more significant in the latter part of the study period (P < 0.05). On the other hand, there were no significant differences in the quality of UCB between the two groups.
Conclusion:
Once clinicians have become accustomed to the trial UBC sampling bag, this method might be a useful method for collecting UCB for unrelated cord blood transplantation.
