Related Experiment Video

Updated: Apr 12, 2026

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
12:02

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

Published on: January 1, 2016

59.3K

[The expression and significance of adaptin-2 in mice cochlea]

Xiang Gu, Rui Song, Zhiji Chen

    Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Journal of Clinical Otorhinolaryngology Head and Neck Surgery
    |May 14, 2015
    PubMed

    Insights

    Adaptin-2 (AP-2) protein is expressed in mature mouse cochlear hair cells, particularly near synapses. This suggests AP-2 plays a key role in synaptic vesicle endocytosis within hair cells.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Auditory Science

    Background:

    • Hair cells in the cochlea are crucial for hearing.
    • Endocytosis is a vital cellular process for neurotransmission and recycling.

    Purpose of the Study:

    • To investigate the expression pattern of adaptin-2 (AP-2) in the mouse cochlea.
    • To explore the potential role of AP-2 in hair cell endocytosis.

    Main Methods:

    • Laser scanning confocal microscopy was utilized.
    • Immune-fluorescence histochemistry was performed.

    Main Results:

    • Adaptin-2 (AP-2) immunoreactivity was detected in the cytoplasm of inner hair cells in mature mice.
    • AP-2 expression was concentrated in the basal part of hair cells, near the ribbon synapse.

    Conclusions:

    • Adaptin-2 (AP-2) is primarily expressed in the synaptic activity zone of hair cells.
    • This localization suggests AP-2 is involved in synaptic vesicle endocytosis.
    • Findings provide a basis for future research on AP-2's physiological and pathological roles.
    Abstract

    More Related Videos

    Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea07:07

    Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

    Published on: February 21, 2016

    11.1K
    Cochlear Surface Preparation in the Adult Mouse09:51

    Cochlear Surface Preparation in the Adult Mouse

    Published on: November 6, 2019

    18.0K

    Related Concept Videos

    The Cochlea01:13

    The Cochlea

    The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.
    52.7K
    Hair Cells01:22

    Hair Cells

    Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here.
    46.8K
    Catenins01:23

    Catenins

    Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
    Catenins in Cell Junctions
    Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
    3.3K
    Tension Response at Adherens Junctions01:26

    Tension Response at Adherens Junctions

    The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
    α-Catenin as a Mechanosensory Protein
    The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
    4.3K