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Updated: Feb 17, 2026
Isolation of Mouse Peritoneal Cavity Cells
Published on: January 28, 2010
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal
Micely D R Hermida1, Rafaela Malta2, Marcos D P C de S Santos2
1Laboratório de Patologia e Biointervenção, Fundação Oswaldo Cruz, FIOCRUZ-BA, Instituto Gonçalo Moniz, Rua Waldemar Falcão, nº 121, Candeal, Salvador, Bahia, CEP 40296-710, Brazil. micelyhermida@gmail.com.
Insights
Investigating leukocyte populations during mouse peritonitis revealed distinct cell distributions based on size and granularity. While these parameters offer initial analysis insights, cell activation and developmental stages complicate precise segregation.
Area of Science:
- Immunology
- Cell Biology
- Inflammation Research
Background:
- Thioglycollate-induced peritonitis is a model for acute inflammation.
- Leukocyte populations undergo dynamic changes during inflammatory responses.
- Flow cytometry gating strategies are crucial for analyzing immune cell subsets.
Purpose of the Study:
- To investigate the diversity and modulation of leukocyte populations in mouse peritonitis.
- To analyze cell populations based on size and granularity at various time points.
- To assess the utility of size and granularity for initial leukocyte gating.
Main Methods:
- Induction of peritonitis in mice using thioglycollate.
- Collection of peritoneal exudate cells at different time points.
- Flow cytometric analysis of cells based on size and granularity.
Main Results:
- Inflammatory cells segregated into four regions (R1-R4) based on size and granularity.
- T lymphocytes (CD3+) and B lymphocytes (B220+) were found in R1/R2 (small, agranular).
- Macrophages (F4/80+) predominated in R3, with granulocytes (Gr1+) in R3/R4.
- Cell distribution suggests recruitment and activation states influence parameters.
Conclusions:
- Size and granularity provide an initial basis for analyzing and sorting thioglycollate-elicited peritoneal cells.
- Cell developmental stage and activation status can interfere with segregation using only size and granularity.
- Further characterization is needed beyond size and granularity for precise leukocyte subset identification.
Objective:
In this study, we investigate the diversity and modulation of leukocyte populations represented in the gates defined by size and granularity at different time points of thioglycollate-induced peritonitis in mouse.
Results:
The inflammatory cells were distributed into four regions (R1-R4) of a data plot graph defined by cell size and granularity. R1 and R2 contained agranular cells that were small in size and predominately included T (CD3+) lymphocytes along with B (B220+) lymphocytes. Macrophages (F4/80+) were the predominant cells found in the R3 region. However, these cells were present in all regions, albeit at a lower frequency in R1 and R2. Granulocytes (Gr1+) were mainly distributed in R3 and R4. The wide distribution of F4/80+ and Gr1+ cells may reflect the recruitment and activation state of the different macrophage and granulocyte populations. Based on these observations, size and granularity may contribute to an initial step in the analysis and sorting of thioglycollate-elicited peritoneal exudate cells. However, the developmental stage and cell activation state may interfere with cell segregation using size and granularity as parameters.
