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Imaging Flow Cytometry to Assess Antigen-Presenting-Cell Function

Kate A Markey1, Kate H Gartlan2,3

  • 1Memorial Sloan Kettering Cancer Center, New York, New York.

Current Protocols in Immunology
|March 7, 2019
PubMed

Insights

This study introduces imaging flow cytometry to quantify phagocytosis and T cell-APC interactions. This advanced technique enables detailed analysis of antigen-presenting cell (APC) function, overcoming previous quantification challenges.

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Quantifying phagocytosis and imaging the immunological synapse between T cells and antigen-presenting cells (APCs) are crucial for understanding immune responses.
  • These critical aspects of APC biology have historically presented significant quantification challenges.

Purpose of the Study:

  • To describe methods for quantifying phagocytosis and imaging the immunological synapse between T cells and APCs.
  • To highlight the utility of imaging flow cytometry in analyzing APC function.

Main Methods:

  • Utilizes imaging flow cytometry, which combines high-throughput data acquisition with the spatial resolution of microscopy.
  • Enables quantification of fluorescence data at specific cellular locations (cell surface and intracellular compartments).

Main Results:

  • Imaging flow cytometry facilitates the analysis of APC functions, including phagocytosis and synapse formation, in unprecedented detail.
  • Allows for the capture of a large number of events and quantification of fluorescence data, overcoming previous limitations.

Conclusions:

  • Imaging flow cytometry offers a powerful approach to quantitatively analyze complex cellular interactions and functions in immunology.
  • This method provides valuable insights into APC biology that were previously difficult to obtain.

Keywords:
ImageStreamimaging flow cytometryimmunological synapsephagocytosis

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