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Updated: Jul 22, 2025
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Published on: August 7, 2021
The Immunophenotypic Profile of Healthy Human Bone Marrow
Joshua E Lewis1, Christopher B Hergott1
1Department of Pathology, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, USA; Harvard Medical School, 25 Shattuck St, Boston, MA 02115, USA.
Insights
Flow cytometry is essential for diagnosing blood cancers by identifying abnormal cell characteristics. This review details normal blood cell development using flow cytometry to aid in diagnosing hematopoietic neoplasms.
Area of Science:
- Hematology
- Immunophenotyping
- Cell Biology
Background:
- Flow cytometry is crucial for multiparametric characterization of hematopoietic cell immunophenotype.
- Aberrant immunophenotypic patterns are key indicators of hematopoietic neoplasms.
- Understanding normal hematopoiesis is essential for identifying abnormal patterns.
Purpose of the Study:
- To review key features of healthy adult hematopoiesis and lineage specification using flow cytometry.
- To provide diagnosticians with a baseline profile of normal hematopoiesis for comparison with clinical samples.
- To illustrate typical flow cytometry plots for normal hematopoiesis.
Main Methods:
- Literature review of flow cytometry applications in hematology.
- Compilation of characteristic flow cytometry data for normal hematopoietic cells.
- Development of illustrative diagrams of flow cytometry plots.
Main Results:
- Detailed description of normal hematopoietic cell populations and their immunophenotypic profiles.
- Presentation of representative flow cytometry plots for various stages of hematopoiesis.
- Establishment of a reference profile for healthy adult hematopoiesis.
Conclusions:
- Flow cytometry is an indispensable tool for diagnosing hematopoietic neoplasms.
- A thorough understanding of normal hematopoiesis immunophenotype is critical for accurate diagnosis.
- This review provides a valuable resource for comparing clinical samples to normal hematopoiesis profiles.
Abstract:
Flow cytometry enables multiparametric characterization of hematopoietic cell immunophenotype. Deviations from normal immunophenotypic patterns comprise a cardinal feature of many hematopoietic neoplasms, underscoring the ongoing essentiality of flow cytometry as a diagnostic tool. However, understanding of aberrant hematopoiesis requires an equal understanding of normal hematopoiesis as a comparator. In this review, we outline key features of healthy adult hematopoiesis and lineage specification as illuminated by flow cytometry and provide diagrams illustrating what a diagnostician may observe in flow cytometric plots. These features provide a profile of baseline hematopoiesis, to which clinical samples with suspected neoplasia may be compared.
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