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Immunohistochemical study of cellular fibronectin in preretinal membranes
1Department of Ophthalmology, School of Medicine, Fukuoka University, Japan.
Insights
Cellular fibronectin is present in preretinal membranes in proliferative vitreoretinopathy and proliferative diabetic retinopathy. This fibronectin likely contributes to cell proliferation and migration in these conditions.
Area of Science:
- Ophthalmology
- Cell Biology
- Biochemistry
Background:
- Fibronectin presence is noted in preretinal membranes of proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR).
- The specific type of fibronectin, plasma or cellular, within these membranes remains unclear.
Purpose of the Study:
- To determine if cellular fibronectin is present in preretinal membranes.
- To investigate the association of cellular fibronectin with preretinal membrane proliferation.
Main Methods:
- Immunohistochemical analysis using a human cellular fibronectin antibody.
- Examination of preretinal membranes from PVR and PDR patients.
Main Results:
- Cellular fibronectin showed a diffuse distribution within membranes from both PVR and PDR patients.
- Neovascular vessels were localized in PDR membranes.
Conclusions:
- Locally produced cellular fibronectin in preretinal membranes may drive cell proliferation and migration.
- Expression of cellular fibronectin is characteristic of pathologic conditions like PDR and PVR.
Background:
The presence of fibronectin has been shown in preretinal membranes from eyes with proliferative vitreoretinopathy and proliferative diabetic retinopathy. It is not clear, however, which type of fibronectin (plasma or cellular) is present in preretinal membranes.
Methods:
A human cellular fibronectin antibody was used to immunohistochemically determine if cellular fibronectin only demonstrates presence or association with proliferation of preretinal membranes.
Results:
The results revealed a diffuse distribution of cellular fibronectin within membranes extracted from proliferative vitreoretinopathy and proliferative diabetic retinopathy patients, and a localized distribution of neovascular vessels in proliferative diabetic retinopathy membranes.
Conclusion:
Cellular fibronectin produced locally in preretinal membranes may contribute a local, cell-mediated process to cell proliferation or migration and the expression of cellular fibronectin by the cellular constituents of preretinal membranes is unique to this pathologic situation like proliferative diabetic retinopathy and proliferative vitreoretinopathy.
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