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Published on: April 8, 2016
[Correlational study between lymphocyte IgE markers: membrane CD23 (mCD23) and soluble CD23 (sCD23) in an atopic
A Sabbah1, C Buchmann, M G Lauret
1Laboratoire d'Immuno-Allergologie, C.H.U., Angers.
Insights
This study found a strong correlation between membrane CD23 (mCD23) and soluble CD23 (sCD23) levels in atopic patients. These markers, along with IgE and eosinophil levels, increased significantly, highlighting their role in atopy.
Area of Science:
- Immunology
- Allergy Research
Background:
- Immunoglobulin E (IgE) receptors, including low-affinity FcεRII (CD23), play a role in allergic diseases.
- Membrane-bound CD23 (mCD23) and soluble CD23 (sCD23) are key markers related to IgE regulation.
Purpose of the Study:
- To investigate the correlation between mCD23 and sCD23 levels.
- To compare these levels with total IgE, eosinophils, eosinophil cationic protein (ECP), and cytokines IL-2, IL-4, and IL-6 in atopic individuals.
Main Methods:
- Flow cytometry was used to measure membrane CD23 (mCD23).
- Soluble CD23 (sCD23) levels were assessed.
- Measurements of IgE, eosinophils, ECP, IL-2, IL-4, and IL-6 were performed in 78 patients (61 atopic, 17 controls).
Main Results:
- A highly significant correlation was observed between mCD23 and sCD23 levels in atopic patients compared to controls.
- Significant increases in mCD23, sCD23, IL-2, IL-4, IL-6, ECP, eosinophils, and total IgE were found in the atopic group.
- The study confirmed elevated IL-4 in atopy and a correlation between ECP and eosinophil counts.
Conclusions:
- There is a significant correlation between membrane and soluble CD23 levels in atopic individuals.
- Elevated levels of CD23, IgE, eosinophils, ECP, and specific cytokines (IL-4) are characteristic of atopy.
- These findings reinforce the utility of CD23 as a marker in allergic research.
Abstract:
IgE has two types of receptors: high affinity RFc sigma I and low affinity RFc sigma II. The latter may be studied by flow cytometry by the membrane marker CD23 (mCD23), expressed on B and T cells, monocytes, macrophages, Langerhans cells, eosinophils and platelets. Soluble CD23 (sCD23) corresponds with the extracellular C terminal domain of mCD23. The aim of the study was to search for a correlation between the levels of mCD23 and sCD23, comparatively with IgE, eosinophils, eosinophil cationic protein (ECP) and to the cytokines IL2, IL4 and IL6. 78 patients took part in the study, divided into two groups: Group 1 = atopics (n = 61); Group 2 = controls (n = 17). The majority presented with rhinitis, asthma, atopic dermatitis isolated or associated. The results showed a highly significant correlation between mCD23 and sCD23 compared with the control group. There was also a statistically significant increase for mCD23, sCD23, cytokines IL2, IL4 and IL6, mediator ECP in parallel with eosinophils and total IgE. This study confirms amongst other things the rise in IL4 in atopy and the correlation between the level of ECP and that of eosinophils, and that there is an overall correlation between mCD23 and sCD23.
