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In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
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La hiperpolarización del progenitor regula la generación secuencial de subtipos neuronales en el desarrollo de la
Ilaria Vitali1, Sabine Fièvre1, Ludovic Telley1
1Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
Cell
|July 31, 2018
Resumen
Las células cerebrales en desarrollo llamadas progenitores se vuelven más hiperpolarizadas a medida que generan nuevos tipos de neuronas. Este cambio bioeléctrico influye en la expresión génica y el desarrollo neuronal, impactando la diversidad neocortical.
Área de la Ciencia:
- La neurociencia
- Biología del desarrollo
- Biología celular
Sus antecedentes:
- La diversidad neuronal neocortical surge de las divisiones progenitoras secuenciales durante la corticogénesis.
- Los procesos dependientes de la actividad regulan la diferenciación postmitótica de las neuronas y el ensamblaje de circuitos.
- El papel de las propiedades bioeléctricas en las células progenitoras no excitables es en gran medida inexplorado.
Objetivo del estudio:
- Investigar el impacto de los procesos bioeléctricos en los progenitores de la zona ventricular durante el desarrollo neocortical del ratón.
- Determinar cómo los cambios en el potencial de la membrana progenitora influyen en el destino celular y la producción neuronal.
Principales métodos:
- Manipulación experimental in vivo del potencial de la membrana progenitora (hiperpolarización) en el neocórtex en desarrollo del ratón.
- Análisis de los programas de transcripción de los progenitores y los modos de división.
- Evaluación de las características laminarias, moleculares, morfológicas y de circuitos de la progenie neuronal.
Principales resultados:
- Los progenitores de la zona ventricular exhiben hiperpolarización progresiva durante la generación sucesiva de subtipos de neuronas.
- La hiperpolarización experimental indujo un cambio a un programa de desarrollo posterior en los progenitores.
- Esto incluyó la generación precoz de progenitores intermedios y la diferenciación neuronal avanzada, mediada por la inhibición de la vía de la Wnt-beta-catenina.
Conclusiones:
- Las propiedades bioeléctricas de la membrana de los progenitores son reguladores críticos de la progresión temporal en los programas de desarrollo.
- La hiperpolarización influye en los estados de transcripción y modos de división del progenitor, impactando la diversidad de las neuronas neocorticales.
- Este estudio revela un nuevo mecanismo que vincula la bioelectricidad a las vías moleculares que controlan la corticogénesis y el destino celular.
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