プロジェニター・ハイパーポラライゼーションは,発達中のニューロンのサブタイプの連続的な生成を調節する
Ilaria Vitali1, Sabine Fièvre1, Ludovic Telley1
1Department of Basic Neurosciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland.
Cell
|July 31, 2018
まとめ
発達中の脳細胞は ニューロンの新しいタイプを 生み出すことで より多極化します この生物電気的変化は遺伝子発現と神経細胞の発達に影響を与え 新皮質の多様性に影響を与えます
科学分野:
- 神経科学
- 発達生物学
- 細胞生物学
背景:
- 新皮質のニューロン多様性は,皮質形成の過程における連続的な祖先分裂から生じる.
- 活動に依存するプロセスは,神経細胞の分化と回路の組み立てを制御する.
- 興奮できない原始細胞における生物電気的性質の役割はほとんど未知のものである.
研究 の 目的:
- ネズミの新皮質発達の過程で心室帯原体に生じる生物電気的過程の影響を調査する.
- プロジェニター膜の潜在変化が細胞の運命とニューロンの出力にどのように影響するかを決定する.
主な方法:
- 発達中のマウスの新皮質におけるプロジェニター膜ポテンシャル (ハイパーポラライゼーション) の実験操作.
- 祖先の転写プログラムと分裂モードの分析
- ニューロンの子孫のラミナー,分子,形状,回路の特徴の評価.
主要な成果:
- 静脈帯の祖先は,連続したニューロンサブタイプ生成の間に進行的な超極化を示す.
- 実験的なハイパーポラライゼーションは 先生の後の発達プログラムへの移行を誘導した.
- これは,Wnt-β-catenin経路の阻害により早期の中間原始細胞生成と高度なニューロン分化を含む.
結論:
- 祖先の生物電気膜の性質は 発達プログラムにおける 時間の進行を制御する重要な要素である.
- ハイパーポラライゼーションは,原始細胞の転写状態と分裂モードに影響し,新皮質のニューロン多様性に影響します.
- この研究は 細胞の皮質形成と運命を制御する 分子経路と 生物電気性を結びつける 新しいメカニズムを明らかにしています
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