トランスクリプションの終結とRNAポリメラーゼのインチワミングの間の結合
E Nudler1, M Kashlev, V Nikiforov
1Public Health Research Institute, New York, New York 10016, USA.
Cell
|May 5, 1995
まとめ
RNAポリメラーゼの動きは,交互にステップを交互に伴う. Oligo(T) は,インチワーム,ストレイン,RNAの放出を調整することにより,信号終止を辿り,転写終止の重要なステップを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 遺伝学 遺伝学とは
背景:
- *E. coliのRNAポリメラーゼは,転写中に単調でインチワームのような動きを交互に繰り返す.
- *インクワームサイクルには,DNA/RNAのストレインとリラクゼーションが含まれ,特徴的な足跡の飛躍につながります.
研究 の 目的:
- * 転写終結におけるオリゴ (T) 経路の役割を調査する.
- *RNAポリメラーゼインチワミングのメカニズムとその終結との関係を解明する.
主な方法:
- *トランスクリプション・ターミネーターにおけるオリゴ (T) 経路の機能を研究した.
- * 分裂に欠陥のあるEcoRIタンパク質を,インチワームの飛躍を抑制する障害物として利用した.
- * 終結部位でのRNA鎖の放出に対する障害物の影響を評価した.
主要な成果:
- * オリゴ (T) トラクタは,インチワームシグナルとして機能し,ジャンプと終了を同期します.
- *EcoRIタンパク質抑制RNA放出でインチワームの飛躍を遮断する.
- *RNAポリメラーゼのストレインとリラクゼーションが終結に不可欠であることを示した.
結論:
- * オリゴー (T) 経路は,RNAポリメラーゼのインチワミングと転写終了を調整するための重要な信号です.
- * インチワーム化中の三元複合体の緊張とリラックスが,終結メカニズムにおける不可欠なステップです.
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