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相关概念视频

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

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This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

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This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
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Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
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DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

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RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

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We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
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相关实验视频

Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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拉曼注射激光是一个激光.

Mariano Troccoli1, Alexey Belyanin, Federico Capasso

  • 1Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA. troccoli@deas.harvard.edu

Nature
|February 25, 2005
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的半导体注射拉曼激光. 这种电动驱动装置消除了对外部的需求,显著提高了对紧的红外光源的拉曼增益.

更多相关视频

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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
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相关实验视频

Last Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
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科学领域:

  • 非线性光学是一种非线性光学.
  • 半导体激光器 半导体激光器
  • 红外光子是红外光子.

背景情况:

  • 刺激拉曼散射 (SRS) 可以实现可调节的光源,但通常需要强大的外部激光.
  • 现有的拉曼激光器的增益很低,限制了实际应用.
  • 量子级联激光器 (QCL) 在中红外线提供电和光学增益.

研究的目的:

  • 开发一种半导体注射拉曼激光,克服传统拉曼源的局限性.
  • 为了实现高拉曼增益和高效率,无需外部光学送.
  • 为了创建紧的,波长敏捷的中和远红外光源.

主要方法:

  • 在量子级联激光器的活性区域内利用三重共振刺激的拉曼散射.
  • 将量子级联激光器集成为内部光学,使电动驾驶成为可能.
  • 演示了一种半导体注射拉曼激光,在没有外部激光接的情况下运行.

主要成果:

  • 与传统的拉曼激光相比,拉曼增益实现了数量级的增强.
  • 证明了高转换效率和激光操作的低值.
  • 成功实现了一种电驱动的,紧的拉曼激光源.

结论:

  • 开发的半导体注射拉曼激光器在现有技术上取得了重大进展.
  • 该设备规避了对外部激光的需求,简化了操作和缩小了尺寸.
  • 它为新一代紧,可调和,高效的中远红外半导体光源铺平了道路.