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相关概念视频

COP Coated Vesicles00:59

COP Coated Vesicles

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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
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Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Coat Assembly and GTPases01:33

Coat Assembly and GTPases

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Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
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Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

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After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
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Transport Across the Golgi01:26

Transport Across the Golgi

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While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
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Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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相关实验视频

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In vivo and in vitro Studies of Adaptor-clathrin Interaction
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In vivo and in vitro Studies of Adaptor-clathrin Interaction

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货物调节了克拉斯林涂层坑的动态.

Manojkumar A Puthenveedu1, Mark von Zastrow

  • 1Department of Psychiatry, University of California at San Francisco, 600 16th Street, San Francisco, CA 94158, USA.

Cell
|October 5, 2006
PubMed
概括
此摘要是机器生成的。

调节的G蛋白合受体 (GPCRs) 细胞内核细胞分解使用特定的克拉涂层 (CCPs) 的子集. 这种CCP的货物特定调节允许在内细胞通路中进行功能专业化.

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 克拉特林涂层坑 (CCP) 是关键的内细胞机器.
  • 中央对手通常被视为一个统一的人口处理多样化的膜货物.
  • 规范货物分离和CCP动态的具体机制仍然不完全理解.

研究的目的:

  • 为了调查特定的货物,如G蛋白结合受体 (GPCRs),是否利用不同的CCP子集进行内细胞分裂.
  • 为了确定含有GPCR的CCP是否具有独特的功能性质.
  • 根据货物的含量,阐明控制中央对手动态的监管机制.

主要方法:

  • 活细胞成像用于观察CCP动态和货物贩运.
  • 生物化学试验分析蛋白质-蛋白质相互作用,包括PDZ-domain介导的链接.
  • 扰乱研究,以评估货物对CCP组装和拆卸的影响.

主要成果:

  • 调节的GPCRs内细胞分裂最好通过一个专门的CCPs子集发生.
  • 含有GPCR的CCP表现出延长的表面停留时间,由载荷-动因细胞骨相互作用调节.
  • 这些专业的CCP显示了延迟的发动机招募,并且可以经历失败的事件,将外套拆卸与货物内部化解开.

结论:

  • 内细胞载荷,如GPCRs,可以分离成不同的CCP子集.
  • CCP动态的货物依赖调节为功能专业化提供了动力机制.
  • 这种专业化优化了早期的内细胞通路,并减轻了不同类型货物的竞争.