Cdc14B和APC/C可以应对DNA损伤.
Peter De Wulf1, Rosella Visintin
1Department of Experimental Oncology, European Institute of Oncology, IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy.
Cell
|July 30, 2008
概括
发芽酵母细胞周期调节器Cdc14,APC/C(Cdh1) 和Plk1已经在人类细胞中重新连接. 这个网络现在控制细胞循环,以应对DNA损伤.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 发芽酵母中的线粒脱离是细胞循环的关键过程.
- 关键的监管机构包括Cdc14,APC/C(Cdh1) 和Plk1.
- 了解这些调节剂对于细胞循环控制至关重要.
研究的目的:
- 为了研究发芽酵母在人体细胞中的线粒离子退出调节者的作用.
- 为了确定蛋白质网络是否被保存或重新连接在人类细胞周期控制中.
- 探索这个网络对人类细胞中DNA损伤的反应.
主要方法:
- 在酵母和人类细胞中的蛋白质网络的比较分析.
- 研究Cdc14,APC/C(Cdh1) 和Plk1在人类细胞周期中的功能.
- 评估DNA损伤诱导后的细胞周期进展.
主要成果:
- 在人类细胞中,Cdc14,APC/C(Cdh1) 和Plk1的网络已经重新连接.
- 这种重新连接的网络控制细胞循环的进展,以应对DNA损伤.
- 证明了细胞循环调节的保存但适应的机制.
结论:
- 涉及Cdc14,APC/C(Cdh1) 和Plk1的细胞周期控制网络是可适应的.
- 人类细胞利用这个网络的重新连接版本来对DNA损伤作出反应.
- 这一发现突显了基本生物过程中的进化可塑性.
相关概念视频
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
02:17Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...