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指标位移测试作为分子计时器

Andrey Buryak1, Friederike Zaubitzer, Alexei Pozdnoukhov

  • 1Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland.

Journal of the American Chemical Society
|August 6, 2008
PubMed
概括

动态Rh染料复合物为跟踪化学反应提供了一种新的方法. 使用这些复合物的紫外光谱学可以揭示诸如腺三酸盐 (ATP) 和腺二酸盐 (ADP) 添加等事件的历史.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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