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相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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相关实验视频

Updated: Jun 28, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

时间解析的RNA SHAPE化学

Stefanie A Mortimer1, Kevin M Weeks

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.

Journal of the American Chemical Society
|November 13, 2008
PubMed
概括
此摘要是机器生成的。

这项研究介绍了RNA折叠动态的时间解析的SHAPE化学. 它揭示了RNA的三级结构是在两个步骤中形成的,长距离相互作用限制了整体折叠率.

更多相关视频

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

相关实验视频

Last Updated: Jun 28, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 结构生物学 结构生物学

背景情况:

  • 通过原料扩展 (SHAPE) 分析的选择性2'-基化提供了RNA结构的单核酸分辨率.
  • 现有的SHAPE方法适用于平衡结构,但对动力学研究是有限的.
  • 了解RNA折叠动态对于阐明生物功能至关重要.

研究的目的:

  • 开发时间解析的SHAPE化学,用于RNA折叠的动力学研究.
  • 为了研究RNA折叠过程中三级相互作用的阶段性形成.
  • 确定RNase P特异性域RNA折叠中的速度限制步骤.

主要方法:

  • 扩展SHAPE化学使用化基架进行快速运动测量 (大约. 一秒钟的快照).
  • 应用时间解析的SHAPE来监测RNase P特异性域RNA的折叠.
  • 分析核酸的灵活性和反应性,以推断随时间推移的结构变化.

主要成果:

  • 在RNase PRNA域中的三级相互作用以两种不同的动态步骤形成.
  • 当地三级接触形成的速度明显快于远程相互作用.
  • 整体折叠的速度受到远距离相互作用的同时形成的限制,而不是非本地结构的破坏.

结论:

  • 时间解析的SHAPE化学能够在单核酸分辨率下轻松研究RNA折叠的动力学.
  • RNA折叠涉及不同的阶段,长距离三级接触的形成是关键的速度限制步骤.
  • 这种方法具有广泛的潜力,用于研究RNA结构动力学和各种生物环境中的结构变化.