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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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相关实验视频

Updated: May 10, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

核糖体分析提供了证据,证明大型非编码RNAs不编码蛋白质.

Mitchell Guttman1, Pamela Russell, Nicholas T Ingolia

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA. mguttman@caltech.edu

Cell
|July 2, 2013
PubMed
概括

大型非编码RNAs (lncRNAs) 对于细胞调节至关重要. 新的研究表明,仅仅是核糖体占用并不证明翻译,这表明大多数lncRNAs不会产生蛋白质.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • 大型非编码RNAs (lncRNAs) 是细胞过程中的关键调节者.
  • 有证据表明,lncRNAs直接作为RNA分子而不是蛋白质产物.
  • 最近的研究表明,核糖体与lncRNAs结合,引发了关于它们的蛋白质编码潜力的疑问.

研究的目的:

  • 为了确定核糖体占用率是否是一个可靠的指标蛋白质编码潜力在lncRNAs.
  • 开发一种可靠的方法来区分编码和非编码的转录.
  • 澄清 lncRNAs 在细胞调节中的功能作用.

主要方法:

  • 对不同RNA类 (lncRNAs,经典非编码RNAs,5' UTRs) 的核糖体占用率进行比较分析.
  • 开发和应用一种新型指标,该指标是基于在停止编码子时的核糖体释放.
  • 测量标准的验证,以区分蛋白质编码和非编码的转录.

主要成果:

  • 核糖体占用不足以将转录分类为编码或非编码.
  • 经典的非编码RNAs和5' UTRs具有与lincRNAs相似的核糖体占用率.
  • 基于新型止码子的度量准确地区分了编码与非编码的转录.

更多相关视频

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

相关实验视频

Last Updated: May 10, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

  • 大多数 lncRNAs 似乎没有被转化为功能性蛋白质.

    • 结论:

    • 单独的核糖体结合是蛋白质翻译的不可靠标记.
    • 停止依赖编码子释放机制是编码潜力的更准确的区分器.
    • 这些发现强烈表明,大多数大型跨基因非编码RNA (lincRNAs) 不通过蛋白质产品起作用.
    • 这项研究阐明了 lncRNAs 在细胞调节中的功能机制.