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在人类细胞中进行基因组规模的CRISPR-Cas9淘汰查

Ophir Shalem1,2, Neville E Sanjana1,2, Ella Hartenian1

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, MA 02142, USA.

Science (New York, N.Y.)
|December 17, 2013
PubMed
概括

这项研究引入了一个基因组规模的CRISPR-Cas9淘汰 (GeCKO) 库,用于高效的基因功能查. 这项技术成功地在人类细胞中识别了必需的基因和耐药性因素,展示了其对生物发现的潜力.

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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