酵母酵母中的环林类蛋白质的基本G1功能
H E Richardson1, C Wittenberg, F Cross
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|December 22, 1989
概括
发芽酵母细胞循环调节剂,循环蛋白 (CLN1,CLN2,CLN3),对于G1阶段的进展至关重要. 它们的功能,可能是激活Cdc28激酶,在生物合成停止后迅速衰减,使细胞增殖成为可能.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 最初因细胞周期周期性而在海洋无脊椎动物中被发现的环素是细胞分裂的关键调节者.
- 三个S. cerevisiae基因 (CLN1,CLN2,DAF1/WHI1) 与环林具有同质性,并与细胞周期进展有关.
研究的目的:
- 研究CLN1,CLN2和DAF1/WHI1基因产物在S. cerevisiae细胞周期进展中的基本功能.
- 为了确定Cln蛋白功能的相位特异性和调节.
- 为了测试Cln蛋白激活Cdc28蛋白激酶的假设.
主要方法:
- 基因删除研究,以评估CLN1,CLN2和DAF1/WHI1损失对细胞周期进展的影响.
- CLN1的条件表达式,以定义其基本功能的时间和持续时间.
- 在生物合成停止后,Cln蛋白功能衰变的分析.
主要成果:
- 突变性消除CLN1,CLN2和DAF1/WHI1导致细胞循环停止,而它们的表达允许增殖.
- Cln 蛋白的基本功能仅限于细胞周期的 G1 阶段.
- 在它们的合成停止后,Cln蛋白的功能迅速衰变,这表明其半衰期很短.
结论:
- 在S. cerevisiae中,Cln蛋白对G1阶段的进展至关重要.
- 这些数据支持了Cln蛋白激活Cdc28蛋白激酶的假设,这对于G1到S过渡至关重要.
- 由于与CLN1和CLN2的功能冗余,DAF1/WHI1被更名为CLN3.
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