来自肠道微生物群的本地细菌调节宿主血清的生物合成
Jessica M Yano1, Kristie Yu1, Gregory P Donaldson1
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Cell
|April 11, 2015
概括
肠道微生物,特别是形成子的细菌,调节胃肠道中血清素 (5-基三胺,5-HT) 的产生. 这种相互作用影响宿主生理学,包括肠道运动性和血小板功能.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 身体生理学 身体生理学
背景情况:
- 胃肠道容纳了身体中大量的血清素 (5-基三胺,5-HT).
- 控制肠道衍生的5-HT代谢的机制尚未完全理解.
- 肠道微生物群在调节宿主5-HT中的作用仍然是积极研究的领域.
研究的目的:
- 研究肠道微生物群在调节宿主血清素 (5-HT) 代谢中的作用.
- 为了确定影响胃肠道中5-HT生物合成的特定微生物因素.
- 了解微生物群依赖于5-HT调节的生理影响.
主要方法:
- 研究了来自小鼠和人类微生物群的原生子形成细菌 (Sp) 的作用.
- 评估了对微生物刺激的反应中来自结肠内染色细胞 (ECs) 的5-HT生物合成.
- 分析了便代谢物及其对体外和无菌小鼠5-HT水平的影响.
- 评估了依赖微生物群的5-HT对胃肠机动性和血小板功能的影响.
主要成果:
- 发现子形成细菌 (Sp) 能够促进来自结肠内染色胺细胞 (ECs) 的5-HT生物合成.
- 表明对肠道5-HT的微生物群依赖性影响显著影响宿主生理学,调节肠道运动性和血小板功能.
- 特定的便代谢物被确定,增加了sp,在氨酸细胞培养物中提升了5-HT,这表明微生物直接向EC发出信号.
- 提高特定微生物代谢物的光度增加了无菌小鼠的结肠和血液5-HT.
结论:
- 子形成细菌是肠道中宿主血清素 (5-HT) 代谢的关键调节者.
- 主体微生物群相互作用在调节涉及5-HT的生物过程中发挥着根本性的作用.
- 微生物代谢物直接向色胺细胞发出信号,影响全身5-HT水平和宿主生理学.
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