皮肤微生物组:当前的技术,挑战和未来的方向
Tasha M Santiago-Rodriguez1, Brice Le François2, Jean M Macklaim1
1Diversigen, Inc., New Brighton, MN 55112, USA.
Microorganisms
|June 15, 2023
概括
皮肤微生物群,包括各种微生物,保护身体,但可以引起疾病. 采样和分析的技术挑战阻碍了对皮肤微生物群的理解.
科学领域:
- 微生物学 微生物学
- 皮肤病学 皮肤病学
- 基因组学就是基因组学.
背景情况:
- 皮肤拥有复杂的细菌,真菌,古生物和病毒微生物组,对于宿主防御和免疫相互作用至关重要.
- 这种皮肤微生物群因体位,遗传和环境因素而异,可能包括机会性病原体.
- 了解皮肤微生物组在健康和疾病中的作用至关重要,文化独立的方法显著推进了研究.
研究的目的:
- 审查皮肤微生物组研究的技术挑战,包括样本采集和处理.
- 讨论目前用于皮肤微生物组分析的测序方法的优缺点.
- 确定皮肤微生物组研究领域的未来研究方向.
主要方法:
- 审查关于皮肤微生物组采样,处理和分析技术的现有文献.
- 批判性评估文化依赖和文化独立的方法,特别是高通量测序.
- 分析影响皮肤微生物组组成的因素及其对健康和疾病的影响.
主要成果:
- 皮肤样本中微生物生物质量低和宿主DNA含量高存在重大技术障碍.
- 当前的收集,提取和分析方法引入了偏见,影响了研究结果.
- 高通量测序极大地扩大了知识,但受限于与样本相关的挑战.
结论:
- 解决样本采集和处理的技术挑战对于推动皮肤微生物组研究至关重要.
- 优化测序方法和最大限度地减少偏差对于准确地描述皮肤微生物群至关重要.
- 未来的研究应该专注于克服目前的局限性,以充分阐明皮肤微生物组在健康和疾病中的作用.
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