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在多个时间点和细胞类型中研究单细胞RNA-seq中的高度可变基因的计算工作流

Jantarika Kumar Arora1, Anunya Opasawatchai2, Sarah A Teichmann3

  • 1Doctor of Philosophy Program in Biochemistry (International Program), Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

STAR protocols
|June 28, 2023
PubMed
概括

本研究引入了一种计算方法,用于分析单细胞RNA测序数据中的高度可变基因 (HVGs). 它有助于了解不同免疫细胞和病毒感染期间的时间点在生物途径中的基因表达动态.

关键词:
生物信息学是一种生物信息学.基因表达 基因表达免疫学 免疫学 免疫学在RNAseqqq中使用.一个单细胞的单细胞.系统生物学 系统生物学

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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