Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Habitat Fragmentation02:31

Habitat Fragmentation

17.6K
Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition.
17.6K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

37.2K
Overview
37.2K
Homologous Recombination02:31

Homologous Recombination

50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.6K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

3.2K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can...
3.2K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Conserved shifts in sperm small non-coding RNA profiles during mouse and human aging.

The EMBO journal·2026
Same author

Dominant-negative NARS1 R534∗ mutation causes wild-type subunit poisoning and heterodimer predominance in cells.

The Journal of biological chemistry·2025
Same author

Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2.

RNA (New York, N.Y.)·2025
Same author

A human histidyl-tRNA synthetase splice variant therapeutic targets NRP2 to resolve lung inflammation and fibrosis.

Science translational medicine·2025
Same author

Molecular basis of human nuclear and mitochondrial tRNA 3' processing.

Nature structural & molecular biology·2025
Same author

Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2.

bioRxiv : the preprint server for biology·2024

相关实验视频

Updated: Jul 14, 2025

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
12:27

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

7.0K

tRNA的更新:通过碎片化的重生.

Bernhard Kuhle1, Qi Chen2, Paul Schimmel3

  • 1Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA; Department of Cellular Biochemistry, University Medical Center Göttingen, Göttingen, Germany.

Molecular cell
|October 6, 2023
PubMed
概括

转移RNAs (tRNAs) 对于蛋白质合成至关重要,并且除了翻译之外还有新的作用. tRNA碎片化产生具有独特生物功能的tRNA衍生小RNA (tsRNA),突出显示tRNA的更新.

关键词:
基于人工智能的RNA结构预测结构 RNA 结构 RNA 结构生物复杂性的生物复杂性.深度学习是一种深度学习.进化 演化 演化 演化 演化 演化 演化 演化非正规的tRNA功能这是一个tRNARNA.在tRNA片段中.由tRNA衍生的小RNA.

更多相关视频

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

12.9K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.2K

相关实验视频

Last Updated: Jul 14, 2025

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations
12:27

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

7.0K
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

12.9K
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
13:37

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

18.2K

科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 生物化学 生物化学

背景情况:

  • 转移RNA (tRNA) 具有独特的结构,对于通过抗三核酸解码mRNA至关重要.
  • tRNAs通过特定的3D形状和序列签名与氨基酸-tRNA合成酶和核糖体相互作用.
  • 除了翻译之外,tRNAs还可以作为信号分子,与其他RNA和蛋白质相互作用.

研究的目的:

  • 探索tRNA碎片化的概念,作为一个创造性的进化过程,产生功能分子.
  • 研究tRNA衍生小RNAs (tsRNAs) 的生物作用和相互作用潜力.
  • 要突出tRNA"renovatio"在生物复杂性和制药应用中的意义.

主要方法:

  • 使用新兴方法来确定完整的tRNA/tsRNA序列和修改.
  • 采用技术研究RNA结构及其三维布局.
  • 整合人工智能 (AI) 驱动的预测,进行全面的分析.

主要成果:

  • tRNA碎片化是一种'娱乐行为',产生特定的tsRNA,而不是随机降解.
  • tsRNAs表现出意想不到的生物功能,利用它们的线性序列和新的3D结构.
  • 这些发现说明了tRNA'renovatio',即tRNA分子的更新和重生.

结论:

  • 测序,结构生物学和人工智能的进步将使tRNAs的详细调查成为可能.
  • 了解tRNA碎片化产物及其相互作用是发现新的生物功能的关键.
  • 这项研究为了解生物复杂性和推进制药工程开辟了新的途径.