使用立即按需下降技术的高通量质谱分析与开放端口采样接口相结合
Vilmos Kertesz1, Dana L Carper1, John F Cahill1
1Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
Rapid communications in mass spectrometry : RCM
|January 11, 2024
概括
一个新的软件增强了立即按需下降 (I.DOT) 技术,用于高通量质谱 (MS) 的开放端口采样接口 (OPSI). 这种自动化系统实现了快速样本分析,改善了药物发现和反应监测.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 自动化技术自动化技术
背景情况:
- 具有开放端口采样接口 (OPSI) 的即时按需下降 (I.DOT) 技术的吞吐量受到软件通信的限制.
- 高通量质谱 (MS) 分析对于药物发现等应用至关重要.
研究的目的:
- 开发使用I.DOT/OPSI-MS合的灵活样本选择和最大化分析吞吐量的新型软件.
- 克服当前I.DOT/OPSI系统的局限性,以进行增强的MS分析.
主要方法:
- 开发了一种新型软件,用于从96个井板中灵活选择样本.
- 使用I.DOT/OPSI与MS相结合,用于高通量分析.
- 通过使用2,3-氨酸甲 (DAN) 和酸盐进行化学反应监测.
主要成果:
- 实现了1.54秒/样本 (7.41分钟/96-井板) 的最大吞吐量.
- 在测试的采样策略中观察到10 ± 1%的相对标准偏差.
- 成功监测了DAN对2,3-纳夫托醇 (NAT) 的化学反应,每个样本的吞吐量为2秒.
结论:
- 开发了一个非接触式的,自动化的I.DOT/OPSI-MS系统,显著提高了吞吐量.
- 该系统的速度和精度使其适用于药物发现和现场反应监测.
- 优化软件界面是实现增强分析能力的关键.
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