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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...

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相关实验视频

Updated: May 11, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

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使用单分子超分辨率显微镜可视化DNA损伤和修复.

Sophie T B Morgan1, Donna R Whelan1, Ashley M Rozario1

  • 1La Trobe Institute for Molecular Science, La Trobe Rural Health School, La Trobe University, Bendigo, VIC, Australia.

Methods in cell biology
|February 15, 2024
PubMed
概括

超高分辨率显微镜可视化DNA损伤部位的DNA修复蛋白. 这种技术允许详细调查细胞中DNA损伤反应 (DDR) 途径.

科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜技术 显微镜技术
  • 分子生物学分子生物学

背景情况:

  • 光显微镜的衍射受限,阻碍了对细胞过程的详细研究.
  • 单分子超分辨率显微镜通过克服衍射极限,提供更高的空间分辨率.
  • 了解DNA损伤反应 (DDR) 途径对于细胞健康和疾病研究至关重要.

研究的目的:

  • 为研究DNA损伤反应 (DDR) 概述超分辨率显微镜测试.
  • 为了研究DNA修复蛋白在受损焦点的时空组织.
  • 量化新生的DNA和DDR蛋白质的局部化.

主要方法:

  • 使用单分子超分辨率显微镜来实现超出衍射极限的分辨率.
  • 在S相同步的不朽细胞系上使用破坏DNA的药物.
  • 结合5-乙烯基-2'-脱氧氨 (EdU) 脉冲标记来跟踪新生的DNA合成.
  • 执行免疫标记以检测特定的DNA损伤反应 (DDR) 蛋白质.

主要成果:

  • 超高分辨率显微镜可可视化单个光体排放,提供高空间分辨率.
  • 这些分析允许详细调查DNA损伤反应 (DDR) 事件.
关键词:
对DNA损伤的反应反应DNA双链断裂是什么意思超高分辨率显微镜的显微镜.dSTORM 这是一个风暴.

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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Last Updated: May 11, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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  • 局部化分析量化了新生DNA和DDR蛋白质在不同的时间点之间的空间关系.

    • 结论:

    • 超高分辨率显微镜是一种强大的工具,用于剖析DNA修复的时空动态.
    • 这些测试为详细调查DNA损伤反应 (DDR) 机制提供了一个框架.
    • 这些发现有助于更深入地了解细胞如何修复DNA损伤.